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1

The real-time polymerase chain reaction

Mikael Kubista, José Manuel Andrade, Martin Bengtsson, Amin Forootan, Jiri Jonák, Kristina Lind, Radek Sindelka, Robert Sjöback, Björn Sjögreen, Linda Strömbom, Anders Ståhlberg, Neven Zoric

Journal:

Molecular Aspects of Medicine

Year:

2006

Language:

english

File:

PDF, 597 KB

english, 2006

2

Pre-analytical processes in medical diagnostics: new regulatory requirements and standards

Dagher, Georges, Becker, Karl-Friedrich, Bonin, Serena, Foy, Carole, Gelmini, Stefania, Kubista, Mikael, Kungl, Penelope, Oelmueller, Uwe, Parkes, Helen, Pinzani, Pamela, Riegman, Peter, Schröder, Ulr

Journal:

New Biotechnology

Year:

2019

Language:

english

File:

PDF, 428 KB

english, 2019

3

Absorption and fluorescence properties of fluorescein

Robert Sjöback, Jan Nygren, Mikael Kubista

Journal:

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

Year:

1995

Language:

english

File:

PDF, 902 KB

english, 1995

4

Natural variation explains most transcriptomic changes among maize plants of MON810 and comparable non-GM varieties subjected to two N-fertilization farming practices

Anna Coll, Anna Nadal, Rosa Collado, Gemma Capellades, Mikael Kubista, Joaquima Messeguer, Maria Pla

Journal:

Plant Molecular Biology

Year:

2010

Language:

english

File:

PDF, 310 KB

english, 2010

5

Absorption flattening in the optical spectra of liposome-entrapped substances

Pernilla Wittung, Johan Kajanus, Mikael Kubista, Bo G. Malmström

Journal:

Year:

1994

Language:

english

File:

PDF, 386 KB

english, 1994

6

Analysis of In Vitro and In Vivo Characteristics of Human Embryonic Stem Cell-Derived Neural Precursors

Kozubenko, Nataliya, Turnovcova, Karolina, Kapcalova, Miroslava, Butenko, Olena, Anderova, Miroslava, Rusnakova, Vendula, Kubista, Mikael, Hampl, Ales, Jendelova, Pavla, Sykova, Eva

Journal:

Cell Transplantation

Year:

2010

Language:

english

File:

PDF, 22.25 MB

english, 2010

7

Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)

Forootan, Amin, Sjöback, Robert, Björkman, Jens, Sjögreen, Björn, Linz, Lucas, Kubista, Mikael

Journal:

Biomolecular Detection and Quantification

Year:

2017

Language:

english

File:

PDF, 542 KB

english, 2017

8

Decoding the Transcriptional Response to Ischemic Stroke in Young and Aged Mouse Brain

Androvic, Peter, Kirdajova, Denisa, Tureckova, Jana, Zucha, Daniel, Rohlova, Eva, Abaffy, Pavel, Kriska, Jan, Valny, Martin, Anderova, Miroslava, Kubista, Mikael, Valihrach, Lukas

Journal:

Year:

2020

File:

PDF, 24.04 MB

2020

9

Cautionary note on contamination of reagents used for molecular detection of SARS-CoV-2

Huggett, Jim F, Benes, Vladimir, Bustin, Stephen A, Garson, Jeremy A, Harris, Karthyn, Kammel, Martin, Kubista, Mikael, McHugh, Timothy D, Moran-Gilad, Jacob, Nolan, Tania, Pfaffl, Michael W, Salit, M

Journal:

Clinical Chemistry

Year:

2020

File:

PDF, 534 KB

2020

10

The interactions between the fluorescent dye thiazole orange and DNA

Jan Nygren, Nicke Svanvik, Mikael Kubista

Journal:

Year:

1998

Language:

english

File:

PDF, 303 KB

english, 1998

11

Characterization of fluorescein–oligonucleotide conjugates and measurement of local electrostatic potential

Robert Sjöback, Jan Nygren, Mikael Kubista

Journal:

Year:

1998

Language:

english

File:

PDF, 148 KB

english, 1998

12

DNA orientation in shear flow

Tomas Simonson, Mikael Kubista

Journal:

Year:

1993

Language:

english

File:

PDF, 698 KB

english, 1993

13

Long-range interactions between DNA-bound ligands

Per Samuelson, Karl Jensen, Mikael Kubista

Journal:

Journal of Molecular Recognition

Year:

1994

Language:

english

File:

PDF, 864 KB

english, 1994

14

Light-Up Probes: Thiazole Orange-Conjugated Peptide Nucleic Acid for Detection of Target Nucleic Acid in Homogeneous Solution

Nicke Svanvik, Gunnar Westman, Dongyuan Wang, Mikael Kubista

Journal:

Analytical Biochemistry

Year:

2000

Language:

english

File:

PDF, 138 KB

english, 2000

15

Detection of PCR Products in Real Time Using Light-up Probes

Nicke Svanvik, Anders Ståhlberg, Ulrica Sehlstedt, Robert Sjöback, Mikael Kubista

Journal:

Analytical Biochemistry

Year:

2000

Language:

english

File:

PDF, 73 KB

english, 2000

16

Quantitative spectral analysis of multicomponent equilibria

Mikael Kubista, Robert Sjöback, Jan Nygren

Journal:

Analytica Chimica Acta

Year:

1995

Language:

english

File:

PDF, 396 KB

english, 1995

17

A new method for the analysis of correlated data using procrustes rotation which is suitable for spectral analysis

Journal:

Chemometrics and Intelligent Laboratory Systems

Year:

1990

Language:

english

File:

PDF, 595 KB

english, 1990

18

The electronically excited states of 2-phenylindole

Bo Albinsson, Svante Eriksson, Reidar Lyng, Mikael Kubista

Journal:

Chemical Physics

Year:

1991

Language:

english

File:

PDF, 813 KB

english, 1991

19

A circular dichroism study of mitochondrial transhydrogenase from beef heart

Bengt Persson, Jan Rydström, Mikael Kubista

Journal:

Biophysical Chemistry

Year:

1991

Language:

english

File:

PDF, 503 KB

english, 1991

20

Procrustes rotation in analytical chemistry, a tutorial

Jose Manuel Andrade, Marı́a P. Gómez-Carracedo, Wojtek Krzanowski, Mikael Kubista

Journal:

Chemometrics and Intelligent Laboratory Systems

Year:

2004

Language:

english

File:

PDF, 327 KB

english, 2004

21

Thermodynamics study of the dimerization equilibria of rhodamine B and 6G in different ionic strengths by photometric titration and chemometrics method

Jahanbakhsh Ghasemi, Ali Niazi, Mikael Kubista

Journal:

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

Year:

2005

Language:

english

File:

PDF, 315 KB

english, 2005

22

Spectrophotometric and thermodynamic study on the dimerization equilibrium of ionic dyes in water by chemometrics method

Ali Niazi, Ateesa Yazdanipour, Jahanbakhsh Ghasemi, Mikael Kubista

Journal:

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy

Year:

2006

Language:

english

File:

PDF, 363 KB

english, 2006

23

An automated procedure to predict the number of components in spectroscopic data

Abdalla Elbergali, Jan Nygren, Mikael Kubista

Journal:

Analytica Chimica Acta

Year:

1999

Language:

english

File:

PDF, 502 KB

english, 1999

24

Determination of Protolytic Constants by Trilinear Fluorescence Spectroscopy

Mikael Kubista, Ismail Hassan Ismail, Amin Forootan, Björn Sjögreen

Journal:

Journal of Fluorescence

Year:

2004

Language:

english

File:

PDF, 1012 KB

english, 2004

25

Monitoring Differentiation of Human Embryonic Stem Cells Using Real-Time PCR

Karin Noaksson, Neven Zoric, Xianmin Zeng, Mahendra S. Rao, Johan Hyllner, Henrik Semb, Mikael Kubista, Peter Sartipy

Journal:

Year:

2005

Language:

english

File:

PDF, 305 KB

english, 2005

26

Determination of equilibrium constants by chemometric analysis of spectroscopic data

Kubista, Mikael., Sjoeback, Robert., Albinsson, Bo.

Journal:

Analytical Chemistry

Year:

1993

Language:

english

File:

PDF, 576 KB

english, 1993

27

Unambiguous Characterization of a Single Test Sample by Fluorescence Spectroscopy and Solvent Extraction without Use of Standards

Nygren, Jan, Elbergali, Abdalla, Kubista, Mikael

Journal:

Analytical Chemistry

Year:

1998

Language:

english

File:

PDF, 247 KB

english, 1998

28

Analysis of correlated spectral data

Scarminio, Ieda., Kubista, Mikael.

Journal:

Analytical Chemistry

Year:

1993

Language:

english

File:

PDF, 796 KB

english, 1993

29

Characterization of a Single Sample by Combining Thermodynamic and Spectroscopic Information in Spectral Analysis

Nygren, Jan, Andrade, José Manuel, Kubista, Mikael

Journal:

Analytical Chemistry

Year:

1996

Language:

english

File:

PDF, 174 KB

english, 1996

30

Structural transitions of chromatin at low salt concentrations: a flow linear dichroism study

Kubista, Mikael, Haerd, Torleif, Nielsen, Peter E., Norden, Bengt

Journal:

Year:

1985

Language:

english

File:

PDF, 873 KB

english, 1985

31

Development and evaluation of three real-time immuno-PCR assemblages for quantification of PSA

Kristina Lind, Mikael Kubista

Journal:

Journal of Immunological Methods

Year:

2005

Language:

english

File:

PDF, 266 KB

english, 2005

32

Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization

Asgeir Brevik, Vendula Rusnakova, Nur Duale, Hege Holte Slagsvold, Ann-Karin Olsen, Ritsa Storeng, Mikael Kubista, Gunnar Brunborg, Birgitte Lindeman

Journal:

Reproductive Toxicology

Year:

2011

Language:

english

File:

PDF, 954 KB

english, 2011

33

Multiway real-time PCR gene expression profiling in yeastSaccharomyces cerevisiaereveals altered transcriptional response ofADH-genes to glucose stimuli

Anders Ståhlberg, Karin Elbing, José Manuel Andrade-Garda, Björn Sjögreen, Amin Forootan, Mikael Kubista

Journal:

Year:

2008

Language:

english

File:

PDF, 963 KB

english, 2008

34

Identification and characterization of genomic nucleosome-positioning sequences

Hans R Widlund, Hui Cao, Stina Simonsson, Elisabet Magnusson, Tomas Simonsson, Peter E Nielsen, Jason D Kahn, Donald M Crothers, Mikael Kubista

Journal:

Journal of Molecular Biology

Year:

1997

Language:

english

File:

PDF, 391 KB

english, 1997

35

TGGA repeats impair nucleosome formation

Hui Cao, Hans R. Widlund, Tomas Simonsson, Mikael Kubista

Journal:

Journal of Molecular Biology

Year:

1998

Language:

english

File:

PDF, 537 KB

english, 1998

36

Flow linear dichroism supports an accordion model for the salt-induced condensation of chromatin

Mikael Kubista, Peter E. Nielsen, Bengt Nordén

Journal:

Biochemical Pharmacology

Year:

1988

Language:

english

File:

PDF, 192 KB

english, 1988

37

Implementation of a proficiency testing for the assessment of the preanalytical phase of blood samples used for RNA based analysis

Kalle Günther, Francesca Malentacchi, Paolo Verderio, Sara Pizzamiglio, Chiara Maura Ciniselli, Ales Tichopad, Mikael Kubista, Ralf Wyrich, Mario Pazzagli, Stefania Gelmini

Journal:

Clinica Chimica Acta

Year:

2012

Language:

english

File:

PDF, 841 KB

english, 2012

38

Detection of immune cell response to M. tuberculosis–specific antigens by quantitative polymerase chain reaction

Ilona Bibova, Irena Linhartova, Ondrej Stanek, Vendula Rusnakova, Mikael Kubista, Miloslav Suchanek, Martina Vasakova, Peter Sebo

Journal:

Diagnostic Microbiology and Infectious Disease

Year:

2012

Language:

english

File:

PDF, 748 KB

english, 2012

39

Prostate-specific antigen mRNA and protein levels in laser microdissected cells of human prostate measured by real-time reverse transcriptase–quantitative polymerase chain reaction and immuno–quantitative polymerase chain reaction

Pamela Pinzani, Kristina Lind, Francesca Malentacchi, Gabriella Nesi, Francesca Salvianti, Donata Villari, Mikael Kubista, Mario Pazzagli, Claudio Orlando

Journal:

Human Pathology

Year:

2008

Language:

english

File:

PDF, 961 KB

english, 2008

40

Spatial expression profiles in the Xenopus laevis oocytes measured with qPCR tomography

Radek Sindelka, Monika Sidova, David Svec, Mikael Kubista

Journal:

Year:

2010

Language:

english

File:

PDF, 482 KB

english, 2010

41

Statistical aspects of quantitative real-time PCR experiment design

Robert R. Kitchen, Mikael Kubista, Ales Tichopad

Journal:

Year:

2010

Language:

english

File:

PDF, 378 KB

english, 2010

42

Quality control for quantitative PCR based on amplification compatibility test

Ales Tichopad, Tzachi Bar, Ladislav Pecen, Robert R Kitchen, Mikael Kubista, Michael W. Pfaffl

Journal:

Year:

2010

Language:

english

File:

PDF, 409 KB

english, 2010

43

Gene expression profiling – Clusters of possibilities

Anders Bergkvist, Vendula Rusnakova, Radek Sindelka, Jose Manuel Andrade Garda, Björn Sjögreen, Daniel Lindh, Amin Forootan, Mikael Kubista

Journal:

Year:

2010

Language:

english

File:

PDF, 1.44 MB

english, 2010

44

LightUp® probes in clinical diagnostics

Mikael Leijon, Mehrdad Mousavi-Jazi, Mikael Kubista

Journal:

Molecular Aspects of Medicine

Year:

2006

Language:

english

File:

PDF, 285 KB

english, 2006

45

Characterization of interaction between DNA and 4',6-diamidino-2-phenylindole by optical spectroscopy

Kubista, Mikael, Aakerman, Bjoern, Norden, Bengt

Journal:

Year:

1987

Language:

english

File:

PDF, 1.00 MB

english, 1987

46

Conformational differences between latent and active plasminogen activator inhibitor, PAI-1: A spectroscopic study

Stig Boström, Johanna Deinum, Jan-Erik Löfroth, Michael Wirth, Mikael Kubista

Journal:

Thrombosis Research

Year:

1990

Language:

english

File:

PDF, 592 KB

english, 1990

47

Multiwavelength Spectrophotometric Determination of Acidity Constants of Morin in Methanol-Water Mixtures

Niazi, Ali, Yazdanipour, Ateesa, Ghasemi, Jahanbakhsh, Kubista, Mikael

Journal:

Collection of Czechoslovak Chemical Communications

Year:

2006

Language:

english

File:

PDF, 185 KB

english, 2006

48

Linear dichroism spectroscopy of nucleic acids

Norden, Bengt, Kubista, Mikael, Kurucsev, Tomas

Journal:

Quarterly Reviews of Biophysics

Year:

1992

Language:

english

File:

PDF, 6.91 MB

english, 1992

49

Binding of 4',6-diamidino-2-phenylindole (DAPI) to AT regions of DNA: Evidence for an allosteric conformational change

Eriksson, Svante, Kim, Seog K., Kubista, Mikael, Norden, Bengt

Journal:

Year:

1993

Language:

english

File:

PDF, 2.37 MB

english, 1993

50

Interaction of 4',6-diamidino-2-phenylindole (DAPI) with poly[d(G-C)2] and poly[d(G-m5C)2]: evidence for major groove binding of a DNA probe

Kim, Seog K., Eriksson, Svante, Kubista, Mikael, Norden, Bengt

Journal:

Journal of the American Chemical Society

Year:

1993

Language:

english

File:

PDF, 774 KB

english, 1993